Real-time Fluorescence Quantitative PCR Instrument

Product Overview

The real-time fluorescence quantitative PCR instrument is mainly used to run real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time. The experimental process data is plotted as a fluorescence curve and presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and finally a PDF format experimental report can be generated.

Rapid and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples (such as blood, body fluids, etc.) taken from other animals, as well as standard curve, melting curve, genotyping and other analyses of special samples.

Basic Principles

Polymerase chain reaction (PCR) is a molecular biology technique used to amplify and amplify specific DNA fragments. It can be seen as a special DNA amplification in vitro, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and appropriate temperature extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time-saving, and is a major innovation in gene amplification technology. PCR technology can amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.

Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR, and detects the entire PCR process in real time through changes in fluorescence signals during the PCR reaction process. The method uses fluorescent chemicals to monitor the total amount of products after each polymerase chain reaction (PCR) cycle, and quantitatively analyzes specific DNA sequences in the test sample through internal or external reference methods.

Basic Structure

Real time fluorescence quantitative PCR instrument is an instrument for real-time detection of reactions, mainly composed of gene amplification thermal cycle system, fluorescence real-time detection system, microcircuit control system, computer and application software. Two core functional modules are: thermal cycling system and real-time fluorescence detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, optical signal transmission components, fluorescence detection components, and control systems.

Instrument Characteristics

1. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

2. Built in 7-inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

3. Android operating system, simple and intuitive software guidance, even beginners can easily learn. Program can be pre-set to enable one click start of experiments.

4. Marlow high-quality Peltier refrigeration chips, combined with Germany's high-end PT1000 temperature sensor and temperature control mode with electric resistance heating compensation edge, have a maximum heating rate of 6 ℃/s and a maximum cooling rate of 4 ℃/s, greatly reducing experimental time.

5. Adopt an efficient heat dissipation system, fully utilize the characteristics of heat conduction, accelerate the heat dissipation of semiconductors during cooling, and speed up temperature control.

6. Adopting an electric resistance heating compensation edge temperature control mode for better temperature uniformity

7. Powerful software features: Fluorescence based PCR system software; Real time amplification reaction curve function; Real time response curve display of specific specimens; Real time temperature curve; Data analysis function; Automatic judgment function for yin-yang results; Graphic display function, etc.

Application Area

□ Basic scientific research

□ Pathogen detection

□ Animal epidemic detection

□ Meat products adulterated

□ Transgenic testing

□ Food hygiene quarantine

□ Drug development and rational drug use

□ Gene expression

□ Water monitoring

Technical Parameter

Sample capacity: 0.2ml x 8 holes

Sample volume: 20-120ul

Applicable consumables: 0.2ml single tube, 0.2ml x 8-tube

Heating method: High performance TEC semiconductor heating/cooling

Temperature range: 4~99 ℃

Heating rate: maximum 6 ℃/s

Cooling rate: maximum 4 ℃/s

Temperature control accuracy: ± 0.1 ℃

Temperature uniformity: ± 0.25 ℃

Excitation light source: high brightness maintenance free LED

Lighting device: high sensitivity, high signal-to-noise ratio photodiode

Number of channels: single channel

Suitable probe or dye: 470/520 FAM, SYBR Green

Detection sensitivity: capable of detecting single copy genes

Linear detection range: 10 ^ 0~10 ^ 10 copies

Linear correlation coefficient: ≥ 0.999

Test repeatability: CV ≤ 3.0%

Operating interface: Comes with a 7-inch Android operating system

Electrical parameters: 220V/50Hz, 200W

Dimensions: 270 * 240 * 152mm (width * depth * height)

Weight: 4.5kg