Product Overview
The 96 well fluorescence quantitative PCR instrument is mainly used to run real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time. The experimental process data is plotted as a fluorescence curve and presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and finally a PDF format experimental report can be generated.
Rapid and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples (such as blood, body fluids, etc.) taken from other animals, as well as standard curve, melting curve, genotyping and other analyses of special samples.
Basic Principles
The principle of the 96 well fluorescence quantitative PCR instrument: Polymerase chain reaction (PCR) is a molecular biology technique used to amplify and amplify specific DNA fragments. It can be regarded as a special DNA amplification in vitro, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and appropriate temperature extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time-saving, and is a major innovation in gene amplification technology. PCR technology can amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.
Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR, and detects the entire PCR process in real time through changes in fluorescence signals during the PCR reaction process. The method uses fluorescent chemicals to monitor the total amount of products after each polymerase chain reaction (PCR) cycle, and quantitatively analyzes specific DNA sequences in the test sample through internal or external reference methods.
Application Area
□ Basic scientific research
□ Pathogen detection
□ Animal epidemic detection
□ Meat products adulterated
□ Transgenic testing
□ Food hygiene quarantine
□ Drug development and rational drug use
□ Gene expression
□ Water monitoring
Product Features
Adopting advanced thermoelectric refrigeration system for higher heating and cooling efficiency
The unique constant current power supply and 6-zone independent temperature control method fully ensure the stable performance of the system. The light source and optical path design make the fluorescence more realistic and reliable
Independent fluorescent channels reduce crosstalk between different channels
Comprehensive analysis functions combined with custom format report functions meet the needs of customers in different scenarios. The software is simple and intuitive to operate, easy to learn and use
Article address:http://www.hmfoodtesting.com/shfx3/142.html