African Swine Fever Testing Equipment

Product Overview:

The HM-P08 African swine fever testing equipment is mainly used for running real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time, and the experimental data is plotted into a fluorescence curve, which is presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and finally a PDF format experimental report can be generated.

Rapid and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples (such as blood, body fluids, etc.) taken from other animals, as well as standard curve, melting curve, genotyping and other analyses of special samples.

Basic Principle:

The principle of HM-P08 African swine fever testing equipment: Polymerase chain reaction (PCR) is a molecular biology technique used to amplify and amplify specific DNA fragments. It can be seen as a special DNA amplification in vitro, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and appropriate temperature extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time-saving, and is a major innovation in gene amplification technology. PCR technology can amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.

Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR, and detects the entire PCR process in real time through changes in fluorescence signals during the PCR reaction process. The method uses fluorescent chemicals to monitor the total amount of products after each polymerase chain reaction (PCR) cycle, and quantitatively analyzes specific DNA sequences in the test sample through internal or external reference methods.

Basic Structure:

The African swine fever rapid detection instrument is a real-time detection reaction instrument, mainly composed of a gene amplification thermal cycle system, a fluorescence real-time detection system, a microcircuit control system, a computer, and application software. Two core functional modules are: thermal cycling system and real-time fluorescence detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, optical signal transmission components, fluorescence detection components, and control systems.

Characteristic:

1. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

2. Built in 7-inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

3. Marlow high-quality Peltier refrigeration chips, combined with Germany's high-end PT1000 temperature sensor and temperature control mode with electric resistance heating compensation edge, have a heating rate of 7 ℃ and a cooling rate of 5 ℃, greatly reducing experimental time.

4. The whole board adopts a 3-second fast lighting mode to ensure the consistency of the hole positions in the experimental results.

5. Simple and intuitive software guidance, making it easy to start detection experiments.

Application Areas:

□ Basic scientific research

□ Pathogen detection

□ Animal epidemic detection

□ Meat products adulterated

□ Transgenic testing

□ Food hygiene quarantine

□ Drug development and rational drug use

□ Gene expression

□ Water monitoring

Technical Parameters:

Sample capacity: 8x0.2ml, supports 8-tube connection

Applicable consumables: Common transparent PCR consumables, 8x0.2ml tube, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/cooling module: imported semiconductor thermoelectric module

Temperature control range: 4 ° C-99 ℃

The average rate of temperature rise and fall is ≥ 2 ° C/second

Temperature control accuracy: ≤± 0.1 ° C

Temperature uniformity: ≤± 0.2 ° C

Number of temperature control areas: Multiple points (2 points)

Number of gradients: 0

Gradient temperature range: none

Number of gradient holes: none

Excitation light source: maintenance free LED

Excitation light wavelength range: 400-700nm

Testing component: Imported photoelectric detector

Number of detection channels: standard configuration 1 channel (FAM), high-end configuration (optional) (FAM, VIC)

Applicable dyes and probes: FAM/SYBR Green I, VIC/HEX/CY3 (optional), ROX/Texas Red (optional), Cy5, TAMARA (optional)

Software function: Fluorescence quantitative PCR system software; Real time amplification reaction curve function; Real time response curve display of specific specimens; Data analysis function; Automatic judgment function for yin-yang results; Graphic display function.

Noise:<45 dB

Screen size: 7 inches (HD)

Touch screen: capacitive type

External USB: Supports data import and export

Hot cover: automatic pressure regulation

Exterior dimensions: 290 (W) * 308 (L) * 130 (H)

Box size: 75 cm in length, 38 cm in width, and 19 cm in height

Net weight: approximately 3Kg