Swine Fever Tester

Product Overview:

The HM-P48 swine fever tester is mainly used for running real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time, and the experimental data is plotted into fluorescence curves, which are presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and finally a PDF format experimental report can be generated.

Rapid and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples (such as blood, body fluids, etc.) taken from other animals, as well as standard curve, melting curve, genotyping and other analyses of special samples.

Basic Principle:

The principle of HM-P48 swine fever tester: Polymerase chain reaction (PCR) is a molecular biology technique used to amplify and amplify specific DNA fragments. It can be regarded as a special DNA amplification in vitro, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and appropriate temperature extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time-saving, and is a major innovation in gene amplification technology. PCR technology can amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.

Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR, and detects the entire PCR process in real time through changes in fluorescence signals during the PCR reaction process. The method uses fluorescent chemicals to monitor the total amount of products after each polymerase chain reaction (PCR) cycle, and quantitatively analyzes specific DNA sequences in the test sample through internal or external reference methods.

Basic Structure:

The African swine fever PCR detector is a real-time reaction detection instrument, mainly composed of a gene amplification thermal cycle system, a fluorescence real-time detection system, a microcircuit control system, a computer, and application software. Two core functional modules are: thermal cycling system and real-time fluorescence detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, optical signal transmission components, fluorescence detection components, and control systems.

Application Areas:

□ Basic scientific research

□ Pathogen detection

□ Animal epidemic detection

□ Meat products adulterated

□ Transgenic testing

□ Food hygiene quarantine

□ Drug development and rational drug use

□ Gene expression

□ Water monitoring