Product Overview:
The African swine fever detector is mainly used to run real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time. The experimental process data is plotted as a fluorescence curve and presented in real-time on the instrument display interface. The experimental data is fitted and analyzed, and finally a PDF format experimental report can be generated.
Basic Principle:
Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR, and detects the entire PCR process in real time through changes in fluorescence signals during the PCR reaction process. The method uses fluorescent chemicals to monitor the total amount of products after each polymerase chain reaction (PCR) cycle, and quantitatively analyzes specific DNA sequences in the test sample through internal or external reference methods.
The African swine fever detector is a real-time reaction detection instrument, mainly composed of a gene amplification thermal cycle system, a fluorescence real-time detection system, a microcircuit control system, a computer, and application software. Two core functional modules are: thermal cycling system and real-time fluorescence detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, optical signal transmission components, fluorescence detection components, and control systems.
Application Areas:
□ Basic scientific research
□ Pathogen detection
□ Animal epidemic detection
□ Meat products adulterated
□ Transgenic testing
□ Food hygiene quarantine
□ Drug development and rational drug use
□ Gene expression
□ Water monitoring
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